I haven't yet, but will have in a couple of days as I have a 6yr. old red tripod coming in who is spay incontinent. She currently is on DES 2x a week. That's where I have a problem. I talked to my vet about DES, and they won't prescribe it, haven't for a while. According to them, it is a synthenic hormone (sp) that can cause breast cancer. So, it looks like she will be going on Proin which has to be given every day. Anyone have experience with Proin?