Hi Laura,

I can tell you from experience that the adventure may not be over. If your bitch is pregnant, you will then have to DNA test all of the potential parents and all of the puppies. Since you have used half brothers, it is entirely likely that the initial 14 DNA markers that AKC uses for determining parentage may not be enough and you may have to got to the second round of 4 additional markers. If that is not sufficient, and it will depend on how closely the half brothers mothers are related and the role of the genetic dice, you will have to go to another source for additional DNA marker testing.

I hope the two males are genetically different enough that you do not have to go there. Good Luck.