Dobemom2b

I know that the vwd test via elisa is seen as outdated since the dna test came along. I have read how there is a overlap of results with many thought to be carriers turning out to be affected when tested via dna.

My question is how reliable is a test which shows a clear result done through Cornell University. Would parents reported clear by them be produce pups clear when later tested by vetgen? How reliable is the test when it shows a clear result?

Linda H

Someone more knowledgeable will surely come along. But a dna test is a dna test and if Cornell U said it was clear then vetgen should report the same results. Clear parents will produce clear pups. A dna test should be as reliable as you can get whether from Cornell or vetgen. They would have to get one sample totally switched with another sample for it not to be reliable. Or maybe a degraded sample.

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dobebug

dobermom,

in the last several years i've seen claims that cornell university did a dna test on someones doberman. in each case where the owner pursued the business further it has turned out that cornell's test was NOT a dna test but an elisa assay.

the only lab licensed to to do the genetic test for vWD in north america is vet gen. the only other lab who was doing dna tests had to cease when they lost a court case to vet gen which was based on the fact that the test is protected by patent & the patent holder have the right to restrict testing to licensee's.

i'm not sure exactly what the wording is but i suspected some while ago that cornell (who does do elsa testing) has something in their paperwork which makes owners & vet clinics who send samples for testing to believe that they are getting a gene test & not a blood assay. there are a whole lot of vets who don't know there is a gene test & continue to rely on the elisa assay for accurate results.

as far as gene test errors go. it's is possible to have a testing error--sloppy lab techniques can account for them & in one case some bad reagents (test chemicals) caused errors but there have been no errors in quite a few years now.

i don't know who is still thinking that cornell's test is a gene test but i'd advise they check further into this.

Dobemom2b

Thank you, I had assumed it was a elisa test done at Cornell however the breeder still feels it gives absolute results since the result is clear through them. In checking online the only thing I could find was on the vetgen site a writeup about a dog named Holly that died and how she did turn out to be clear via vetgen. Her parents had clear elisa tests and the author basically said he had never heard of false clears.

I guess that would have been a clearer question, have elisa clears ever been shown to be unreliable results when the same dogs or cleared by heritage pups were dna tested.

I just don't have a lot of faith in elisa tests even with no more than I know about it.

(it was my error in forming the original question, I had assumed the CU test was elisa per the topic title but mistakingly said dna in the original post, the breeder in question did not seem to know there was a difference between the CU test and the vetgen dna test)

whitecat

i think the answer to your question is yes elisa clears have been shown to be unreliable when the offspring were tested thru dna by vet gen. this happened when the dna test started to be used and breeders found out some of their dogs who had been declared clear by elisa actually weren't clear genetically. the elisa test is only a snapshot in time of the clotting factor at the moment the blood is drawn, it has uses, but it doesn't tell you the genetic status of the dog.

Murreydobe

Absolutely, this happened to me with my first litter, born shortly before the dna test was available. I bred a clear via ELISA test bitch to a clear by ELISA test stud dog. Five months later the dna test came on the market, and we found out some of the resulting puppies were affected via dna.

There's more of a problem with the ELISA test than just an overlap of categories. The results can also be affected by a wide variety of external factors-hormones, stress level, etc. The results aren't worth the paper they're printed on.

As Dobebug said, Vetgen is the ONLY entity who can legally offer dna tests for vWD. This is because they hold the patent on the actual genetic location of the vWD mutation.

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ProMod

I have a question. I was offered a pup that was tested for VwD during treatment for a broken leg. The test came back positive for VwD. It was the assay test and NOT vet-Gen.

Can the broken leg, abandonment by jerk owner affect the VwD results? By how much?
(The fracture was three days or so old at time of testing.)

The dog has it's dew claws removed, tail docked AND EARS done!
Vet claims dog "developed VwD at 5 1/2 months of age" is why it survived all previous medical proceedures WITHOUT ANY COMPLICATIONS WHATSOEVER.

Am I being jerked around here or what's the deal?

Megs

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Murreydobe

[quote=Megs;168104][color="Navy"][

Even if this dog is affected, with 2 bad gene copies, he is not clinically affected, because he has gone through docking, ear-cropping, etc. without any complications.

QUOTE]

The amount of available von Willebrands Factor is at a high point in puppies-dogs tend to make less of it as they mature and age. So not having a clotting problem during docking, ear cropping, etc. isn't particularly significant. Certainly not any kind of proof a problem won't appear in the future.

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dragonman

It is very important for our readership to know that VetGen holds the patten on vWd testing for our breed and that their are different types of vWd. There is a company out there called Pinpoint DNA Technologies out of Atlanta GA advertising their Pawsitive ID program claiming to do several canine and feline disease testing including vWd in on full panel but on careful inspection of the breeds covered by their testing you will see that dobermans are NOT included especially for the vWD and the hypothyroidism test (which is for congenital hypothyroidism and not the type of hypothyroidism usually seen in dobermans).

I'm going to start a new thread about this because I found out about this company from another DT member who thinks their dog is disease free which might not be the case.

Here's their website on the test available and the breeds covered.
Pawsitive ID | Home

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